====== Fluorescence and z-stacking ======

When you display slides that contain fluorescent (multi channel) data, or when the slide represents a z-stack, additional selection controls automatically appear in the viewport to allow you to interact with specific file format features as needed.

=== Fluorescent data ===

When fluorescent data is found in a virtual slide, a histogram control panel automatically appears. 

{{ :fluo_controls.png?400 |}}

This allows the end-user to mix and match individual channels. Basic histogram clipping features are possible, too.

=== Z-stack data ===

When multiple planes (z-stack data) is found in a virtual slide, a slidebar for the various layers automatically appears. 

{{ ::z-stacks.png?400 |}}

This allows the end-user seamlessly navigate through a stack. 

=== Combining fluorescence and z-stack data ===

When you have slides that contain both multi-channel data and z-stack planes, both controls appear.

{{ ::fluo_and_z-stack_controls_combined.png?400 |}}

=== More options ===

[[https://www.pathomation.com/pma.studio|PMA.studio]] is Pathomation's flagship viewing environment for all things virtual slide related. 

{{ :fluo_controls_pma_studio.png?400 |}}

When you upgrade from PMA.slidebox to PMA.studio, you gain a range of extra functionality, including histogram clipping and per-channel gamma correction.  [[https://www.pathomation.com/pma.studio|Find out more about PMA.studio at our dedicated webpage]].